Smith-Plt'field meth'od

[John Black burn Smith, English surgeon, contemporary; Robert L. Pittfield, American physician, *i87o For staining flagella: a mordant is made by saturating a hot saturated solution of corrosive sublimate with ammonia alum, then mixing with an equal part of 10 per cent, tannic acid solution and adding one-half part per cent, carbol-fuchsin solution; the preparation is treated with this mixture and then stained with a saturated alcoholic solution of gentian violet, i, in a saturated ammonium alum solution, 10.