staggers

(stag’erz). One of various functional and organic diseases of the brain and spinal cord of domestic animals, known also as blind s., especially See under absolute alcohol: clear with xylol and embed with parafin. Biondi-Heidenhain s.: A stain prepared by mixing a saturated watery solution of orange (, 100 parts; saturated watery solution of acid fuchsin, 20 parts; a saturated aqueous solution of methyl-green, 50 parts. Before staining, dilute with water, 1 part to 100. Biot’s method (jor tubercle bacilli): A very thin spread of sputum is made. It is fixed as usual; stain with warm carbolfuchsin for two or three minutes; without washing, decolorize thoroughly first in 25 per cent. nitric acid, then in absolute alcohol; wash with water; then place in ordinary, concentrated formol for at least three minutes; wash, dry, examine. The bacilli, colored a dark violet, stand out clearly against the unstained background. The detail of their structure comes out well, and the granules ~ of Much take on the same stain as the bacilli themselves and are clearly seen to be of the same nature. Birch-Hirschfeld method (for amyloid): Stain in a 2 per cent. alcoholic solution of Bismarck brown five minutes; wash in absolute alcohol, and then ten minutes in distilled water; stain in 2 per cent. solution of gentian-violet five to ten minutes; wash in a dilute acetic-acid solution; mount in levulose. The amyloid becomes red, the ti ue, brown. Bismarck brown: An anilin stain used in a 1 per cent. aqueous solution or in a saturated solution of 40 per cent. alcohol. blue ma : An injecting material for anatomic and histologic preparations, made by preparing a solution of gelatin warmed to 70° C., and coloring it to the desired shade with an aqueous solution of Berlin blue. Béhmer’s hematoxylin: Hematoxylin crystals, 1 gm.; absolute alcohol, 10 c.c.; pota ium alum, 10 gm.; distilled water, 200 c.c. Di olve the hematoxylin crystals in the alcohol and the alum in the distilled water. While constantly stirring, add the first solution to the second. The whole is then left for about fourteen days in an open jar or dish, protected from the dust, during which time the color changes from violet to blue. After filtering, the stain is ready for use. Sections, either loose or fixed to the slide or cover-slip, are placed in this solution, and after about half an hour are washed with water. If the nuclei are well stained, the further treatment with alcohol may be commenced. Bowhill’s method (for spores): Stain the preparation for ten to fifteen minutes in a solution of 15 c.c. saturated alcoholic solution of orcein, 10 c.c. aqueous solution of tannin, 30 c.c. distilled water, warming slightly. Buchner’s method (for anaérobic cultures): An inoculated slant-tube with a loose cotton stopper is placed in a larger sealed tube, from which the oxygen is absorbed by a mixture of 6 gm. of pyrogallic acid and 1000 c.c. of pota ium hydroxid. The slant-tube is held above the reducing mixture by a bent wire. Buerger’s method (for capsules): The preparation is fixed in Miiller’s fluid, washed in water and in alcohol, covered with iodin for two minutes, washed in alcohol, dried, stained in anal in-water, gentian violet, and washed in 2 per cent. salt solution. Bunge’s mordant: A mordant for staining flagella. consisting of 25 c.c. of solution of ferric chlorid diluted twenty times; 75 c.c. of a saturated aqueous solution of tannic acid. Just before using, a 3 per cent. solution of hydrogen dioxid is added, and the solution is then filtered. Burri’s method: A method of staining organisms, especially spirochetes, with India ink. Cajal’s double method (jor gansglionceis): Α modification of Golgi’s quick method by repeating the steps to get more perfect impregnation. Capaldi’s culture-medium: For the bacillus of tuberculosis fluid agar in tubes is mixed with fresh egg-yolk aseptically obtained, the yolk membrane being seared with a hot knife. The medium is solidified on cooling so as to form slants. carbolfuchsin. See Zichl- Neclson’s carbol- Suchsin. carmin: A section stain used in the following solutions: alcoholic borax-carmin