BIXIN

The red resinous colouring matter of annatto. Bolley and Mylius prepare it by digesting the dried alcoholic extract of annatto with ether; repeatedly treating the least soluble portion (which contains the greater part of the colouring matter) with hot ether; di olving the remainder in alcohol; precipitating the alcoholic solution with lead acetate; decomposing the washed precipitate with sulphuretted hydrogen; extracting the colouring matter therefrom by hot alcohol; and precipitating the alcoholic solution with water.