(med-it-er-a’ne-an)

1. Means. 2. A substance which transmits impu. 3. A substance used in the culture of acteria. Called also culture-m. Abe’s m. (for gonococci), mix 500 gm. of fat-free meat, finely hashed, with ro0o c.c. of water. Allow to stand in ice-chest for from eighteen to twenty-five hours, then filter through paper and a Chamber land filter into sterile containers: for use it is mixed with fluid or solid peptone media in the proportion of 1 to 5. Bruns’ glucose m., a medium for mounting fresh ti ue specimens: made by mixing distilled water, 140 c.c., glucose, 40 gm., and glycerin, 10 c.c., then adding camphorated spirit, 10 c.c., and filtering. clearing m., a medium for rendering histologic specimens transparent. Dieudonné’s m., a culture-medium composed of 30 parts of oxblood, 30 of pota ium hydroxid, and 140 of cholera agar. Drigalski-Conradi m., a _ culture medium made by adding nutrose and crystal violet to lactose litmus agar, using 3 per cent. of agar. The crystal violet inhibits the growth of many bacteria other than the typhoid bacillus. It is used in detecting typhoid bacilli in the blood. Called also Conradi’s m. Elsner’s m., a culture medium made by adding a sterilized pota ium iodid solution of to per cent. strength to Holz’s potato-gelatin. m. of Endo, a culture-medium for typhoid bacilli: to 1 liter of water are added 500 gm. of ground beef, ro gm. of peptone, 5 gm. of sodium chlorid, and 30 gm. of agar. The mixture is boiled, filtered, neutralized, and then made alkaline by 10 c.c. of a 10 per cent. solution of sodium carbonate, then 10 gm. of pure lactose are added, then ς c.c. of alcoholic solution of fuchsin, then 25 c.c. of 10 per cent. solution of sodium sulphite. esculin m., a medium for the isolation of the colon bacillus, containing esculin and iron citrate, in which the colon bacillus produces a black colony. Holz's m., potato-gelatin. L&éffler’s 562 MEGA COCCUS m., a culture-medium far diphtheria bacillus, containing 1 part bouillon, 3 parts blood-serum, and 1 > percent. of glucose. pa ive m., a medium which does not alter the structure of ti ues that are placed in it. Piorkowski’s m., a medium consisting of 100 parts of urine that has undergone ammoniacal fermentation, 0.5 part of peptone, and 3.3 parts of gelatin. Wickersheimer’s m. Sce under fluid.