tuber'culin

A preparation made from cultures of the tubercle bacillus, employed as a means of diagnosis and in the treatment, by active immunization, of tuberculosis. bacilli, obtained by treatment with a decinorraal solution of caustic soda. the pulverized bacilli are not removed by centrifugation, but are allowed to settle as much as they will, after which the supernatant fluid is mixed with equal parts of glycerin. of tubercle bacilli with chloral hydrate and alcohol is called tuherculase; the proce is very complicated the preparation is now recommended solely for the immu-; nizing treatment of tuberculous cattle; (2) tulase is a somewhat similar preparation obtained by treating tubercle bacilli with chlorides; it is employed by v. Behring in the prevention of tuberculosis in children, being given in milk. extracellular toxins, obtained from a bouillon culture of the tubercle bacillus, the intracellular toxins extracted from the protoplasm of the bacilli by phosphoric acid. practically the same as non-concentrated old t. (TO), that is, TO of ^ strength; a similar preparation is made by the manufacturers of Koch's tuberculins, and called by them TOA (^tuberculin original alt) by triturating dried tubercle bacilli with sand, and expre ing under hydraulic pre ure. prepared by precipitating old t. with 94 per cent, alcohol, washing, di olving in water, and filtering; this is again precipitated with alcohol, washed, and dried; it ia prepared in tablets or in aqueous solution, and is used in testing for tuberculosis by means of Calmette's ophthalmoreaction. chloroform, and alcohol to free it from admixture with albuminous and other inert substances. made by treating old tuberculin with peroxide of hydrogen, on the theory that toxins are converted into antitoxins by oxidation. from old tuberculin by treatment with alcohol and bismuth, in order to remove certain injurious substances; to this preparation, he gave the name tuberculocidin; (2) by treatment of the fluid portion of a tubercle-bacillus culture with sodium-bismuth-iodide in acetic acid and absolute alcohol, Klebs obtained a still purer preparation, called an i i pht his in; (3) for use in mixed infections, Klebs made a preparation from a culture of Diplococcus semilunar is, which he called selenin. original], a tuberculin prepared, in the same way as Koch's old tuberculin (TOA), from cultures of bovine tubercle bacilli. sion, and bouillon filtrate in this table. from very virulent cultures by fractional extraction with water, physiological salt solution, and diluted glycerin, under gradually increasing temperature; it is said to contain all the toxic principles existing in the tubercle bacilli. is made of finely triturated tubercle bacilli in distilled water or physiological salt solution, which on centrifugation separates into an upper translucent fluid layer (TO, tuberculin ober) and a muddy sediment (TR, tuberculin rest); the latter is dried, again triturated, emulsified, and centrifugated, and this proce is repeated until only an opalescent fluid is obtained; glycerin is now added in quantity to make a 20 per cent, solution which is then ready for use; this is intended to immunize against the bacilli. culture of tubercle bacilli in 5 per cent, glycerin bouillon, from 4 to 6 weeks old, is filtered and the filtrate is evaporated by heat to its volume; it contains only the soluble bacterial products, and whatever immunity it confers is against the toxins only, not the bacilli. action of a trichophyton (T. holosericum album) upon a culture of tubercle bacilli, rapidly growing and virulent culture, dried, powdered, and extracted with ether; the extract is again dried, powdered, and extracted with distilled water. of bovine tuberculosis, recommended on the ground that its immunizing properties are equal to those of preparations made from cultures of the bacilli of human tuberculosis, while it is much le dangerous. (2) IK (Ger. immun-korper); a preparation made from the blood-corpuscles of animals immunized against both human and bovine tubercle bacilli, the claim being made that the immunizing substances, or antibodies, in tuberculosis are in the blood-cells and not in the serum. similar to v. Behring's tuberculase. washing a culture of tubercle bacilli and treating with strong sulphuric acid to remove the endotoxin; the preparation is then di olved in a large amount of water and the precipitate is collected and dried; this is di olved in a weak alkaline solution, in the proportion of 0.5:100.0. It is claimed that the preparation is without toxic action, but po e es strong immunizing properties. to one-tenth its original volume.