Zalefski's Method

For ascertaining the presence of iron compounds in the liver or other ti ues. The ti ues are treated in bulk instead of in separate sections. The methods are as follows: (a) Ammonium sulphid Method, (i) The pieces of ti ue are left in er cent, alcohol for twenty-four hours. They are then hardened for twenty-four hours in 96 per cent, alcohol, to which a few drops of strong, yellow ammonium sulphid have been added. They must be shaken up from time to time. They are next lardened in absolute alcohol, to which several drops ammonium sulphid are added. They may en be cut. The alcohol should completely fill the ttle; corks must not be used, because they give iron reaction with ammonium sulphid. (b) Potasm-ferrocyanid Method. The pieces are left in per cent, alcohol for twenty-four hours. They then placed for two or three days in a I per cent, lution of pota ium ferrocyanid in 96 per cent, They are then kept in a I per cent, solution pota ium ferrocyanid in 65 per cent, alcohol. ey are now placed in I or 2 per cent, acid alcohol cohol, 96 per cent. for two or three days. e sections may then be cut and may afterward be ined in carmin. Treatment with 65 per cent, alcohol in 3 is employed to aid the penetration of the acid d subsequently. Y ox ferrous compounds pota ium ferrocyanid may be similarly used.