STAIN

n. Stan. Gr., miA i?, <rm'Aos Lat., macula. Fr., tache. Ger., Fleck. 1. A discoloration, a discolored spot or tract of surface, [a, 48.] 2, A dyeing preparation. Blue s. See MAL del pinto. Carbol-Fuchsin s. See ZiehVs s. Ehrlich-Biondi triple s. A mixture of 5 c. c. of a saturated watery solution of methyl green, 10 of a saturated solution of methyl orange, and 2 of Rubin s. (acid fuchsine). The specimen is put into the s. for from 15 minutes to 12 hours, washed in dilute alcohol, then for 1 minute in absolute alcohol, and then in xylol and benzol, and mounted in xylol balsam. The green stains the nuclei, and the acid fuchsine and the orange stain the ground substance of the specimen, [a, 34.] Ehrlich's s. A s. for tubercle bacilli, composed of a saturated solution of aniline oil in distilled water to which is added enough of a saturated alcoholic solution of fuchsine to give the fluid a dark, opalescent appearance. The fluid to be examined is dried on a cover-gla , pa ed through a flame, floated on the coloring fluid, and boiled from 3 to 5 minutes. The specimen is then decolorized by floating it on a 25-per-cent. aqueous solution of nitric acid, rinsed in water or alcohol, and mounted. If, after rinsing, the specimen is floated in a dilute aqueous solution of methylene-blue, all bacteria except the tubercle bacilli are stained blue, the red of the latter contrasting with the blue of the former, [a, 34.] Fried lander's s. The specimen is stained as by Ziehl's method, heated over a flame until steam is given off, washed in distilled water, and decolorized with a 5-per-oent. alcoholic solution of nitric acid, and then a few drops of an aqueous solution of methylene-blue are added as a contrast s. The specimen is then washed and mounted in balsam, [a, 34.] Gabbets's s. A method of staining tubercle bacilli in Ziehl's s. The specimen is then placed in a mixture of 100 c. c. of a 25-per-cent. solution of sulphuric acid and 2 c. c. of methylene-blue, washed, dried, and mounted, [n, 34.] Gibbes's tubercle s. A solution of 2 parts of rosaniline hydrochloride and 1 part of methylene-blue, made by heating and trituration in a gla mortar, with the addition of 3 parts of aniline oil di olved in 15 of rectified spirit, and finally of 15 parts of distilled water added slowly, [a, 34.] Kiihiie's s. A solution of 1'5 part of methylene-blue, 10 parts of absolute alcohol, and 100 of a 5-per-eent. solution of absolute alcohol, in which, in staining bacteria, the section is immersed for half an hour, washed in water, decolorized in a solution of 10 drops of hydrochloric acid in 500 grammes of water, immersed in a solution of 8 drops of a saturated solution of lithium carbonate in 10 grammes of water, washed in distilled water, dipped in absolute alcohol colored with methylene-blue, placed in aniline oil colored with methylene-blue, and washed in pure aniline oil and then in xylol. fa, 34.] Lewis's s. A s. for nerve ti ue. On a frozen section of ti ue a few drops of a 2-per-cent. osmic-acid solution are poured and allowed to remain a few minutes; the section is then washed in water, immersed in a 1-percent, watery solution of aniline-black for one or two hours, and mounted in pota ium acetate or in glycerin, [a, 34.] LBfller's s. A mixture of 30 c. c. of a saturated solution of methylene-blue and 100 c. c. of a l-to-10,000 solution of caustic potash; used for staining bacteria, [a, 34.] Milky s's. See LEUCOPLAKIA. Pregle's s. A modification of Kiihne's s. The alcohol in a dehydrated section is absorbed by a piece of filter paper, and a drop of acetone celloidin solution is placed upon it; the section is then stained by dropping upon it the solution composing Kuhne's s. from a pipette, [a. 34.] Weigert's haematoxylin s. A proce of staining various ti ue elements of the central nervous system. Sections of ti ue that has been hardened in Miiller's fluid are soaked for twenty-four hours in a saturated aqueous solution of neutral cupric acetate diluted with an equal bulk of water. They are then soaked for two hours in a solution of 1 gramme of hsematoxylin crystals in a mixture of 10 c. c. of 97-per-cent. alcohol, 90 c. c. of water, and 1 c. c. of a saturated aqueous solution of lithium carbonate. They are next washed in two or three waters, rinsed in alcohol, and transferred to a bleaching solution of 2'5 grammes of pota ium ferricyanide, 2 grammes of sodium biborate, and 200 c. c. of water, in which they remain until the gray matter has a distinct yellow color and the white matter is bluish-black. The sec A, ape; A2, at; A3, ah; A, all; Ch, chin; Ch2. loch (Scottish); [s. 530]